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superscript vilo complementary dna synthesis kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript vilo complementary dna synthesis kit
    Superscript Vilo Complementary Dna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+vilo+complementary+dna+synthesis+kit/DNA/pmc12576131-144-8-14
    Average 99 stars, based on 1 article reviews
    superscript vilo complementary dna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Loss of endothelial membrane KIT ligand affects systemic KIT ligand levels but not bone marrow hematopoietic stem cells.
    Article Snippet: Two thousand cells were fluorescence-activated cell sorted directly into Trizol Reagent (Invitrogen), and RNA was isolated. .. Reverse transcription was performed using Superscript Vilo complementary DNA synthesis kit (Invitrogen). .. Quantitative polymerase chain reaction (qPCR) was performed with Taqman Gene Expression Assays (Applied Biosystems): total Kitl, Mm00442972_m1; mKitl, Mm01232339_m1; and Hprt, Mm01545399_m1.

    Article Title: DESIGNER FAT CELLS: ADIPOGENIC DIFFERENTIATION OF CRISPR-CAS9 GENOME-ENGINEERED INDUCED PLURIPOTENT STEM CELLS
    Article Snippet: Samples for gene expression analysis were harvested and total RNA was isolated from the cell according to recommendations of the manufacturer (48300; Norgen Biotek, Thorold, ON, Canada). .. Reverse transcription was performed using the superscript VILO complementary DNA synthesis kit (11755500; Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions. .. Quantitative polymerase chain reaction (qPCR) was performed using FASTSybr (4385617; Applied Biosystems, Waltham, MA, USA) following manufacturer’s instructions.

    Article Title: Tissue factor pathway-related biomarkers in liver cancer: activated factor VII–antithrombin complex and tissue factor mRNA levels are associated with mortality
    Article Snippet: The RNA samples were extracted with TRI Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol and quantified by NanoDrop One instrument (Thermo Fisher Scientific) and Qubit fluorometer using the RNA Broad-Range Assay Kit (Thermo Fisher Scientific). .. Reverse transcription was performed using the SuperScript VILO complementary DNA Synthesis Kit (Thermo Fisher Scientific), and evaluation of mRNA expression was performed on the 7500 Real-Time PCR System (Applied Biosystems) using TF TaqMan assays (Hs01076029 m1 F3, Applied Biosystems by Thermo Fisher Scientific) and GAPDH as the housekeeping gene (Hs99999905_m1). ..

    Article Title: Designer Fat Cells: Adipogenic Differentiation of CRISPR-Cas9 Genome-Engineered Induced Pluripotent Stem Cells
    Article Snippet: Samples for gene expression analysis were harvested and total RNA was isolated from the cell according to recommendations of the manufacturer (48300; Norgen Biotek). .. Reverse transcription was performed using the superscript VILO complementary DNA synthesis kit (11755500; Life Technologies) following the manufacturer’s instructions. .. Quantitative polymerase chain reaction (qPCR) was performed using FASTSybr (4385617; Applied Biosystems) following manufacturer’s instructions.

    Article Title: Meniscus gene expression profiling of inner and outer zone meniscus tissue compared to cartilage and passaged monolayer meniscus cells
    Article Snippet: .. Reverse transcription-quantitative polymerase chain reaction RNA samples were reverse transcribed using the SuperScript VILO complementary DNA Synthesis Kit (Catalog # 11,754,050; Life Technologies). .. Quantitative polymerase chain reaction (qPCR) was performed using PowerUP SYBR Green Master Mix (Catalog # A25776; Thermo Fisher Scientific Baltics UAB) and the StepOne Plus Realtime PCR System (Model 4,376,374; Applied Biosystems).

    Article Title: Meniscus gene expression profiling of inner and outer zone meniscus tissue compared to cartilage and passaged monolayer meniscus cells
    Article Snippet: .. RNA samples were reverse transcribed using the SuperScript VILO complementary DNA Synthesis Kit (Catalog # 11,754,050; Life Technologies). .. Quantitative polymerase chain reaction (qPCR) was performed using PowerUP SYBR Green Master Mix (Catalog # A25776; Thermo Fisher Scientific Baltics UAB) and the StepOne Plus Real-time PCR System (Model 4,376,374; Applied Biosystems).

    DNA Synthesis:

    Article Title: Loss of endothelial membrane KIT ligand affects systemic KIT ligand levels but not bone marrow hematopoietic stem cells.
    Article Snippet: Two thousand cells were fluorescence-activated cell sorted directly into Trizol Reagent (Invitrogen), and RNA was isolated. .. Reverse transcription was performed using Superscript Vilo complementary DNA synthesis kit (Invitrogen). .. Quantitative polymerase chain reaction (qPCR) was performed with Taqman Gene Expression Assays (Applied Biosystems): total Kitl, Mm00442972_m1; mKitl, Mm01232339_m1; and Hprt, Mm01545399_m1.

    Article Title: DESIGNER FAT CELLS: ADIPOGENIC DIFFERENTIATION OF CRISPR-CAS9 GENOME-ENGINEERED INDUCED PLURIPOTENT STEM CELLS
    Article Snippet: Samples for gene expression analysis were harvested and total RNA was isolated from the cell according to recommendations of the manufacturer (48300; Norgen Biotek, Thorold, ON, Canada). .. Reverse transcription was performed using the superscript VILO complementary DNA synthesis kit (11755500; Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions. .. Quantitative polymerase chain reaction (qPCR) was performed using FASTSybr (4385617; Applied Biosystems, Waltham, MA, USA) following manufacturer’s instructions.

    Article Title: Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cells.
    Article Snippet: Residual genomic DNA was removed by treatment with DNase I (Sigma-Aldrich). .. First-strand complementary DNA synthesis was carried out using SuperScript VILO complementary DNA Synthesis Kit (Invitrogen) quantitative PCR was performed using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies; 600882) in duplicates. .. Ct values were obtained on an Agilent 8890 Real-Time PCR System, and data were generated with the comparative threshold cycle (Delta CT) method by normalizing to hypoxanthine phosphoribosyltransferase (Hprt).

    Article Title: Tissue factor pathway-related biomarkers in liver cancer: activated factor VII–antithrombin complex and tissue factor mRNA levels are associated with mortality
    Article Snippet: The RNA samples were extracted with TRI Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol and quantified by NanoDrop One instrument (Thermo Fisher Scientific) and Qubit fluorometer using the RNA Broad-Range Assay Kit (Thermo Fisher Scientific). .. Reverse transcription was performed using the SuperScript VILO complementary DNA Synthesis Kit (Thermo Fisher Scientific), and evaluation of mRNA expression was performed on the 7500 Real-Time PCR System (Applied Biosystems) using TF TaqMan assays (Hs01076029 m1 F3, Applied Biosystems by Thermo Fisher Scientific) and GAPDH as the housekeeping gene (Hs99999905_m1). ..

    Article Title: Designer Fat Cells: Adipogenic Differentiation of CRISPR-Cas9 Genome-Engineered Induced Pluripotent Stem Cells
    Article Snippet: Samples for gene expression analysis were harvested and total RNA was isolated from the cell according to recommendations of the manufacturer (48300; Norgen Biotek). .. Reverse transcription was performed using the superscript VILO complementary DNA synthesis kit (11755500; Life Technologies) following the manufacturer’s instructions. .. Quantitative polymerase chain reaction (qPCR) was performed using FASTSybr (4385617; Applied Biosystems) following manufacturer’s instructions.

    Article Title: Meniscus gene expression profiling of inner and outer zone meniscus tissue compared to cartilage and passaged monolayer meniscus cells
    Article Snippet: .. Reverse transcription-quantitative polymerase chain reaction RNA samples were reverse transcribed using the SuperScript VILO complementary DNA Synthesis Kit (Catalog # 11,754,050; Life Technologies). .. Quantitative polymerase chain reaction (qPCR) was performed using PowerUP SYBR Green Master Mix (Catalog # A25776; Thermo Fisher Scientific Baltics UAB) and the StepOne Plus Realtime PCR System (Model 4,376,374; Applied Biosystems).

    Article Title: Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cells
    Article Snippet: Residual genomic DNA was removed by treatment with DNase I (Sigma-Aldrich). .. First-strand complementary DNA synthesis was carried out using SuperScript VILO complementary DNA Synthesis Kit (Invitrogen) quantitative PCR was performed using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies; 600882) in duplicates. .. Ct values were obtained on an Agilent 8890 Real-Time PCR System, and data were generated with the comparative threshold cycle (Delta CT) method by normalizing to hypoxanthine phosphoribosyltransferase ( Hprt ).

    Article Title: Meniscus gene expression profiling of inner and outer zone meniscus tissue compared to cartilage and passaged monolayer meniscus cells
    Article Snippet: .. RNA samples were reverse transcribed using the SuperScript VILO complementary DNA Synthesis Kit (Catalog # 11,754,050; Life Technologies). .. Quantitative polymerase chain reaction (qPCR) was performed using PowerUP SYBR Green Master Mix (Catalog # A25776; Thermo Fisher Scientific Baltics UAB) and the StepOne Plus Real-time PCR System (Model 4,376,374; Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cells.
    Article Snippet: Residual genomic DNA was removed by treatment with DNase I (Sigma-Aldrich). .. First-strand complementary DNA synthesis was carried out using SuperScript VILO complementary DNA Synthesis Kit (Invitrogen) quantitative PCR was performed using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies; 600882) in duplicates. .. Ct values were obtained on an Agilent 8890 Real-Time PCR System, and data were generated with the comparative threshold cycle (Delta CT) method by normalizing to hypoxanthine phosphoribosyltransferase (Hprt).

    Article Title: Tissue factor pathway-related biomarkers in liver cancer: activated factor VII–antithrombin complex and tissue factor mRNA levels are associated with mortality
    Article Snippet: The RNA samples were extracted with TRI Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol and quantified by NanoDrop One instrument (Thermo Fisher Scientific) and Qubit fluorometer using the RNA Broad-Range Assay Kit (Thermo Fisher Scientific). .. Reverse transcription was performed using the SuperScript VILO complementary DNA Synthesis Kit (Thermo Fisher Scientific), and evaluation of mRNA expression was performed on the 7500 Real-Time PCR System (Applied Biosystems) using TF TaqMan assays (Hs01076029 m1 F3, Applied Biosystems by Thermo Fisher Scientific) and GAPDH as the housekeeping gene (Hs99999905_m1). ..

    Article Title: Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cells
    Article Snippet: Residual genomic DNA was removed by treatment with DNase I (Sigma-Aldrich). .. First-strand complementary DNA synthesis was carried out using SuperScript VILO complementary DNA Synthesis Kit (Invitrogen) quantitative PCR was performed using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies; 600882) in duplicates. .. Ct values were obtained on an Agilent 8890 Real-Time PCR System, and data were generated with the comparative threshold cycle (Delta CT) method by normalizing to hypoxanthine phosphoribosyltransferase ( Hprt ).

    SYBR Green Assay:

    Article Title: Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cells.
    Article Snippet: Residual genomic DNA was removed by treatment with DNase I (Sigma-Aldrich). .. First-strand complementary DNA synthesis was carried out using SuperScript VILO complementary DNA Synthesis Kit (Invitrogen) quantitative PCR was performed using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies; 600882) in duplicates. .. Ct values were obtained on an Agilent 8890 Real-Time PCR System, and data were generated with the comparative threshold cycle (Delta CT) method by normalizing to hypoxanthine phosphoribosyltransferase (Hprt).

    Article Title: Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cells
    Article Snippet: Residual genomic DNA was removed by treatment with DNase I (Sigma-Aldrich). .. First-strand complementary DNA synthesis was carried out using SuperScript VILO complementary DNA Synthesis Kit (Invitrogen) quantitative PCR was performed using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies; 600882) in duplicates. .. Ct values were obtained on an Agilent 8890 Real-Time PCR System, and data were generated with the comparative threshold cycle (Delta CT) method by normalizing to hypoxanthine phosphoribosyltransferase ( Hprt ).

    Expressing:

    Article Title: Tissue factor pathway-related biomarkers in liver cancer: activated factor VII–antithrombin complex and tissue factor mRNA levels are associated with mortality
    Article Snippet: The RNA samples were extracted with TRI Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol and quantified by NanoDrop One instrument (Thermo Fisher Scientific) and Qubit fluorometer using the RNA Broad-Range Assay Kit (Thermo Fisher Scientific). .. Reverse transcription was performed using the SuperScript VILO complementary DNA Synthesis Kit (Thermo Fisher Scientific), and evaluation of mRNA expression was performed on the 7500 Real-Time PCR System (Applied Biosystems) using TF TaqMan assays (Hs01076029 m1 F3, Applied Biosystems by Thermo Fisher Scientific) and GAPDH as the housekeeping gene (Hs99999905_m1). ..

    Polymerase Chain Reaction:

    Article Title: Meniscus gene expression profiling of inner and outer zone meniscus tissue compared to cartilage and passaged monolayer meniscus cells
    Article Snippet: .. Reverse transcription-quantitative polymerase chain reaction RNA samples were reverse transcribed using the SuperScript VILO complementary DNA Synthesis Kit (Catalog # 11,754,050; Life Technologies). .. Quantitative polymerase chain reaction (qPCR) was performed using PowerUP SYBR Green Master Mix (Catalog # A25776; Thermo Fisher Scientific Baltics UAB) and the StepOne Plus Realtime PCR System (Model 4,376,374; Applied Biosystems).



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